porozendoОстеопороз и остеопатииOsteoporosis and Bone Diseases2072-26802311-0716Endocrinology Research Centre10.14341/osteo200912-6porozendo-4069Research ArticleСтатьиArticlesАссоциация полиморфного варианта (CA)nгена кальцитонина (CALCA) с риском развитияпостменопаузального остеопороза и уровнемкальцитонина в сыворотке крови у русских женщинВолго-Уральского региона РоссииAssotsiatsiya polimorfnogo varianta (CA)ngena kal'tsitonina (CALCA) s riskom razvitiyapostmenopauzal'nogo osteoporoza i urovnemkal'tsitonina v syvorotke krovi u russkikh zhenshchinVolgo-Ural'skogo regiona RossiiSeleznevaL. I.-KhusainovaR. I.-NurlygayanovR. Z.-FazlyevaE. A.-ZiganshinT. M.-KozhemyakinaE. V.-LesnyakO. M.-KhusnutdinovaE. K.-200915122008121№1 (2009)26Copyright © Selezneva L.I., Khusainova R.I., Nurlygayanov R.Z., Fazlyeva E.A., Ziganshin T.M., Kozhemyakina E.V., Lesnyak O.M., Khusnutdinova E.K., 20092009Selezneva L.I., Khusainova R.I., Nurlygayanov R.Z., Fazlyeva E.A., Ziganshin T.M., Kozhemyakina E.V., Lesnyak O.M., Khusnutdinova E.K.Selezneva L.I., Khusainova R.I., Nurlygayanov R.Z., Fazlyeva E.A., Ziganshin T.M., Kozhemyakina E.V., Lesnyak O.M., Khusnutdinova E.K.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.osteo-endojournals.ru/jour/article/view/4069

Постменопаузальный остеопороз - распространенное многофакторное заболеванием с выраженной генетической предрасположенностью. Целью данной работы явилось изучение ассоциаций полиморфного варианта (CA)n гена кальцитонина (CALCA) c риском развития постменопаузального остеопороза и уровнем кальцитонина в сыворотке крови у русских женщин Волго-Уральского региона России. В работе использованы образцы ДНК 372 женщин в возрасте от 48 до 77 лет (175 с переломами и 197 без переломов). Показатели МПК шейки бедра исследованы у 185, МПК поясничного отдела позвоночника - у 203 женщин, определение уровня кальцитонина в сыворотке крови проведено у 168 женщин. Выявлено восемь аллельных вариантов (CA)n-полиморфизма гена CALCA с числом СА повторов 10, 11, 12, 15, 16, 17, 18 и 19. Генотип CALCA10*10 является протективным в отношении развития ОП (OR = 0,21) и остеопении (OR = 0,24). Обнаружена ассоциация генотипов CALCA10*10 и CALCA17*17 с высоким уровнем МПК шейки бедра. Выявлена статистически значимая ассоциация аллеля CALCA*17 с повышенным уровнем кальцитонина в сыворотке крови.

Postmenopausal osteoporosis is a widespread complex disease with strong genetic predisposition. The aim of this study was to fnd association of (CA)n polymorphism of calcitonin gene (CALCA) with the risk of postmenopausal osteoporosis and serum calcitonin level in Russian women from Volga-Ural region. DNA samples of 372 women aged from 48 to 77 years old (175 with fractures and 197 without fractures) were used in the study. Femoral neck BMD was measured in 185 women, lumbal spine BMD - in 203 women. The serum calcitonin was measured in 168 women. Eight alleles of (CA)n polymorphism were revealed: each allele contained 10, 11, 12, 15, 16, 17, 18 and 19 CA repeats. CALCA*10*10 genotype is a marker of the decreased risk of osteoporosis (OR = 0,21) and osteopenia (OR = 0,21). CALCA*10*10 and CALCA*17*17 genotypes were shown to be associated with high femoral neck BMD. Statistically signifcant association of CALCA*17 allele with the increased calcitonin level have been shown.

постменопаузальный остеопорозкальцитонингенетический полиморфизмpostmenopausal osteoporosiscalcitoningenetic polymorphismReferencesAlevizaki M., Stevenson J.C., Girgis S.I. et al. Altered calcitonin gene in a young patient with osteoporosis // BMJ. 1989. Vol. 6, № 298 (6682). P. 1215-1216.Alevizaki M., Stevenson J.C., Girgis S.I. et al. Altered calcitonin gene in a young patient with osteoporosis // BMJ. 1989. Vol. 6, № 298 (6682). P. 1215-1216.Andrew T., Antioniades L., Scurrah K.J. et al. Risk of wrist fracture in women is heritable and is infuenced by genes that are largely independent of those infuencing BMD // J Bone Miner Res. 2005. Vol. 20, № 1. P. 67-74.Andrew T., Antioniades L., Scurrah K.J. et al. Risk of wrist fracture in women is heritable and is infuenced by genes that are largely independent of those infuencing BMD // J Bone Miner Res. 2005. Vol. 20, № 1. P. 67-74.Beringer T.R., Ardill J., H. M. Taggart. Absence of evidence for a role of calcitonin in the etiology of femoral neck fracture // Calcif Tissue Int. 1986. Vol. 39, № 5. P. 300-303.Beringer T.R., Ardill J., H. M. Taggart. Absence of evidence for a role of calcitonin in the etiology of femoral neck fracture // Calcif Tissue Int. 1986. Vol. 39, № 5. P. 300-303.Braga V., Mottes M., Mirandola S. et al. Association of CTR and COLIA1 alleles with BMD values in peri- and postmenopausal women // Calcif Tissue Int. 2000. Vol. 67, № 5. P. 361-366.Braga V., Mottes M., Mirandola S. et al. Association of CTR and COLIA1 alleles with BMD values in peri- and postmenopausal women // Calcif Tissue Int. 2000. Vol. 67, № 5. P. 361-366.Chesnut 3rd C.H., Baylink D.J, Sisom K. et al. Basal plasma immunoreactive calcitonin in postmenopausal osteoporosis // Metabolism . 1980. Vol. 29, № 6. P. 559-562.Chesnut 3rd C.H., Baylink D.J, Sisom K. et al. Basal plasma immunoreactive calcitonin in postmenopausal osteoporosis // Metabolism . 1980. Vol. 29, № 6. P. 559-562.Drews K., Seremak-Mrozikiewicz A., Bartkowiak-Wieczorek J. et al. Genetic polymorphism of the calcitonin receptor gene and bone mineral density in Polish population of postmenopausal women // Ginekol Pol. 2005. Vol. 76, № 8. P. 612-618.Drews K., Seremak-Mrozikiewicz A., Bartkowiak-Wieczorek J. et al. Genetic polymorphism of the calcitonin receptor gene and bone mineral density in Polish population of postmenopausal women // Ginekol Pol. 2005. Vol. 76, № 8. P. 612-618.Evans R., Marel G., Lancaster E. et al. Bone mass is low in relatives of osteoporosis patients // Ann Intern Med. 1988. Vol. 109. P 870-873.Evans R., Marel G., Lancaster E. et al. Bone mass is low in relatives of osteoporosis patients // Ann Intern Med. 1988. Vol. 109. P 870-873.Kim J.G., Choi Y.M., Moon S.Y., Lee J.Y. Association of the calcitonin gene (CA) polymorphism with bone mass and bone responsiveness to hormone therapy in postmenopausal Korean women // Menopause. 2003. Vol. 10, № 6. P. 544-549.Kim J.G., Choi Y.M., Moon S.Y., Lee J.Y. Association of the calcitonin gene (CA) polymorphism with bone mass and bone responsiveness to hormone therapy in postmenopausal Korean women // Menopause. 2003. Vol. 10, № 6. P. 544-549.Magana J.J., Gomez R., Cisneros B. et al. Association of the CT gene (CA) polymorphism with BMD in osteoporotic Mexican women // Clin Genet. 2006. Vol. 70, № 5. P. 402-408.Magana J.J., Gomez R., Cisneros B. et al. Association of the CT gene (CA) polymorphism with BMD in osteoporotic Mexican women // Clin Genet. 2006. Vol. 70, № 5. P. 402-408.Mathew C.C. The isolation of high molecular weight eucariotic DNA // Methods in molecular biology. Ed Walker J.M. N.Y.; Haman press, 1984. P. 31-34.Mathew C.C. The isolation of high molecular weight eucariotic DNA // Methods in molecular biology. Ed Walker J.M. N.Y.; Haman press, 1984. P. 31-34.Miyao M., Hosoi T., Emi M. et al. Association of bone mineral density with a dinucleotide repeat polymorphism at the calcitonin (CT) locus // J Hum Genet. 2000. Vol. 45, № 6. P. 346-350.Miyao M., Hosoi T., Emi M. et al. Association of bone mineral density with a dinucleotide repeat polymorphism at the calcitonin (CT) locus // J Hum Genet. 2000. Vol. 45, № 6. P. 346-350.Pondel M. Calcitonin and calcitonin receptors: bone and beyond // Int J Exp Pathol. 2000. Vol. 81, № 6. P. 405-422.Pondel M. Calcitonin and calcitonin receptors: bone and beyond // Int J Exp Pathol. 2000. Vol. 81, № 6. P. 405-422.Ralston S. The genetics of osteoporosis // Bone. 1999. Vol. 25, № 1. P. 85-86.Ralston S. The genetics of osteoporosis // Bone. 1999. Vol. 25, № 1. P. 85-86.Reginster J.Y. Calcitonin // Rev. Med. Liege. 1996. Vol. 51, № 1. P. 116-119.Reginster J.Y. Calcitonin // Rev. Med. Liege. 1996. Vol. 51, № 1. P. 116-119.Seeman E., Hopper J., Bach L. et al. Reduced bone mass in daughters of women with osteoporosis // N. Engl. J. Med. 1989. Vol. 320. P. 554-558.Seeman E., Hopper J., Bach L. et al. Reduced bone mass in daughters of women with osteoporosis // N. Engl. J. Med. 1989. Vol. 320. P. 554-558.Sugino K., Kure Y., Iwasaki H., Matsumoto A. Does total thyroidectomy induce metabolic bone disturbance? // Int. Surg. 1992. Vol. 77, № 3. P. 178-180.Sugino K., Kure Y., Iwasaki H., Matsumoto A. Does total thyroidectomy induce metabolic bone disturbance? // Int. Surg. 1992. Vol. 77, № 3. P. 178-180.Tiegs R.D., Body J.J., Wahner H.W. et al. Calcitonin secretion in postmenopausal osteoporosis // N. Engl. J. Med. 1985. Vol. 25, № 312(17). P. 1097-1100.Tiegs R.D., Body J.J., Wahner H.W. et al. Calcitonin secretion in postmenopausal osteoporosis // N. Engl. J. Med. 1985. Vol. 25, № 312(17). P. 1097-1100.Tsukamoto K. A polymorphic CA repeat sequence at the human calcitonin locus // J. Hum. Genet. 1998. Vol. 43, № 2. P. 146-147.Tsukamoto K. A polymorphic CA repeat sequence at the human calcitonin locus // J. Hum. Genet. 1998. Vol. 43, № 2. P. 146-147.Uitterlinden A.G., van Meurs J.B., Rivadeneira F., Pols H.A. Identifying genetic risk factors for osteoporosis // J Musculoskelet Neuronal Interact. 2006. Vol. 6, № 1. P. 16-26.Uitterlinden A.G., van Meurs J.B., Rivadeneira F., Pols H.A. Identifying genetic risk factors for osteoporosis // J Musculoskelet Neuronal Interact. 2006. Vol. 6, № 1. P. 16-26.WHO technical report Assessment of fracture risk and its application to screening for postmenopausal osteoporosis // Geneva, 1994. ser. 843. p. 129.WHO technical report Assessment of fracture risk and its application to screening for postmenopausal osteoporosis // Geneva, 1994. ser. 843. p. 129.Wuster C., Raue F., Meyer C. et al. Long-term excess of endogenous calcitonin in patients with medullary thyroid carcinoma does not affect bone mineral density // J. Endocrinol. 1992. Vol. 134, № 1. P. 141-147.Wuster C., Raue F., Meyer C. et al. Long-term excess of endogenous calcitonin in patients with medullary thyroid carcinoma does not affect bone mineral density // J. Endocrinol. 1992. Vol. 134, № 1. P. 141-147.

The authors declare that there are no conflicts of interest present.