Inhibition of activity of collagen degradation in cartilage of patients with osteoarthrosis byactivation of glycolysis
https://doi.org/10.14341/osteo201118-12
Abstract
Aim. To study the effect of glycolysis activators deferrioxamine (DFO), CoCl2, V(SO4)2 and mimosine on
collagen cleavage activity by collagenase in osteoarthritic (OA) articular cartilage explants.
Materials and methods. 32 OA articular cartilages obtained after arthroplasty were examined in the study. Cartilages were cultured in the presence of 10-50μM DFO, CoCl2, V(SO4)2 or mimosine. Collagen cleavage activity was measured by ELISA. Inhibition of protein or DNA synthesis in the presence of [3H]-labeled proline or thymidine, respectively, was used for evaluation of examined agent toxicity.
Results. Glycolysis activators DFO, CoCl2, V(SO4)2 or mimosine were capable of inhibiting type II collagen cleavage activity in OA articular cartilage explants. The examined agents have shown no toxic effect in the concentrations used.
Conclusion. Glycolysis activation in articular chondrocytes may offer a means of inhibiting articular cartilage destruction in OA patients.
collagen cleavage activity by collagenase in osteoarthritic (OA) articular cartilage explants.
Materials and methods. 32 OA articular cartilages obtained after arthroplasty were examined in the study. Cartilages were cultured in the presence of 10-50μM DFO, CoCl2, V(SO4)2 or mimosine. Collagen cleavage activity was measured by ELISA. Inhibition of protein or DNA synthesis in the presence of [3H]-labeled proline or thymidine, respectively, was used for evaluation of examined agent toxicity.
Results. Glycolysis activators DFO, CoCl2, V(SO4)2 or mimosine were capable of inhibiting type II collagen cleavage activity in OA articular cartilage explants. The examined agents have shown no toxic effect in the concentrations used.
Conclusion. Glycolysis activation in articular chondrocytes may offer a means of inhibiting articular cartilage destruction in OA patients.
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Review
For citations:
Chetina E.V., Chetina E.V. Inhibition of activity of collagen degradation in cartilage of patients with osteoarthrosis byactivation of glycolysis. Osteoporosis and Bone Diseases. 2011;14(1):8-12. (In Russ.) https://doi.org/10.14341/osteo201118-12
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